MetaWRAP analysis of sample #25500 that represents metagenomic determination of the presence of <i>C</i>. <i>infans</i> in the stool sample and provides a demonstration that a standard pipeline for analyzing whole-genome sequencing shotgun metagenomics data can identify multiple <i>Campylobacter</i> species present in the stool sample.

Additionally, those sequence reads can be assembled into partial or complete genomes that can be used for source tracking or studying transmission dynamics unlike other molecular tools. A. Kronogram used to represent/display that 23% of total sequencing reads and 98% of the Campylobacter specific sequencing reads are matching C. infans, and provides a breakdown of the number of sequence reads generated for that sample the match Campylobacter species at the different taxonomic levels. B. Kronogram used to represent/display that when present in high enough concentrations as for this sample, identified Campylobacter species can be partially or completely genome assembled using a standard analysis pipeline and provides a breakdown of the number of assembled reads generated for that sample the match Campylobacter species at the different taxonomic levels. For this sample, 68% of all the assembled reads and 100% of the Campylobacter contigs for this sample were identified as C. infans. C. Shotgun metagenomic analysis pipelines can also rely on software to bin reads/contigs based on various parameters such as GC content of the data. Blobplot of all the assembled contigs that were binned from the sample based on percentage of GC content at the Order level of taxonomic identification, which demonstrates Campylobacterales as the dominate Order in the assembled contigs and that the binning process of the shotgun metagenomic analysis can also identify Campylobacter species from the stool samples. The Campylobacter bin contained 15 contigs that equals 20,789 bp or 1.2% of the genome.