Additional file 2 of Impaired response of blood neutrophils to cell-death stimulus differentiates AQP4-IgG-seropositive NMOSD from MOGAD

Additional file 2: Figure S2. Evaluation of spontaneous and PMA induced ROS production in AQP4 + NMOSD, MOGAD patients and HC granulocytes. Granulocytes from patients and matched HC were isolated and analyzed simultaneously and cultured without (black dots, none) and with PMA (red dots). Intracellular ROS were indirectly assessed by flow cytometry analysis of DHR 123. Depicted is the mean fluorescence intensity (MFI) of oxidized-DHR123. A) Data are represented with lines connecting the unstimulated and PMA-stimulated data points of the same patient sample B) Depicted are the data from unstimulated and MOGAD samples in a different scale to show group differences. Each dot represents an individual subject. HC: n = 17; AQP4 + NMOSD: n = 9; MOGAD: n = 8