We introduced the same CRISPR null deletion as in [7] in the unc-17 and flp-19 reporter alleles syb4491 and syb3278, yielding alleles ot1208 and ot1209. A small Z-stack (6–7 images at .8 micron) around the middle of the worm are shown with 10 μm scale bars. Graphs compare expression in wild type and mutant worms with the number of animals examined listed at the bottom of the bar. P-values were calculated by Fisher’s exact test. (EPS)</p
<p>A. Micrographs of fixed young adult hermaphrodites stained with Oil Red O (top panels) or Sudan B...
A) C. elegans dye uptake assay. In wild type worms, DiI dye is taken up by 6 pairs of amphid (head) ...
<p>(A) <i>Left panel</i>: Single worm duplex PCR was performed on ten animals of the <i>fahd-1(tm500...
unc-39R203Q mutant animals (either canonical e257 allele or CRISPR/Cas9 genome engineered ot1173 all...
Fig 9A:unc-62(e644) mutant animals show a loss of RIC marker expression, including an eat-4 CRISPR r...
A: ASI and AWA analysis in ceh-36(gj2127) and ceh-37(ok642) mutant animals. Markers used are CRISPR/...
<p>(A,B) Strip chart of the brood size of (A) <i>lin-35</i> null worms following <i>efl-1</i>, <i>li...
Fig 3A:ceh-14(ot900) mutant animals show a loss of neuropeptide-encoding gene expression in PVW, inc...
Fig 5A: In a vab-3(ot1243) mutant allele, many neurons in the anterior ganglion lose their NeuroPAL ...
Fig 6A: In ceh-32(ok343) null mutant animals, OLL, URY but also RIA markers are almost always lost (...
<p>Null <i>unc-18</i> (<i>e81</i>) worms were rescued transgenically with wild-type (Wt) <i>unc-18</...
<p>(A, B) Genomic analysis to confirm that single-copy insertion has occurred. (A) Scheme showing th...
International audienceMultiple advances have been made to increase the efficiency of CRISPR/Cas9 edi...
Representative fluorescence images of wild-type, asna-1(ok938), asna-1(ΔHis164) and asna-1(A63V) adu...
<p>(<b>A</b>) Representative western blots showing the expressed full-length Tau (0N4R and 0N3R) and...
<p>A. Micrographs of fixed young adult hermaphrodites stained with Oil Red O (top panels) or Sudan B...
A) C. elegans dye uptake assay. In wild type worms, DiI dye is taken up by 6 pairs of amphid (head) ...
<p>(A) <i>Left panel</i>: Single worm duplex PCR was performed on ten animals of the <i>fahd-1(tm500...
unc-39R203Q mutant animals (either canonical e257 allele or CRISPR/Cas9 genome engineered ot1173 all...
Fig 9A:unc-62(e644) mutant animals show a loss of RIC marker expression, including an eat-4 CRISPR r...
A: ASI and AWA analysis in ceh-36(gj2127) and ceh-37(ok642) mutant animals. Markers used are CRISPR/...
<p>(A,B) Strip chart of the brood size of (A) <i>lin-35</i> null worms following <i>efl-1</i>, <i>li...
Fig 3A:ceh-14(ot900) mutant animals show a loss of neuropeptide-encoding gene expression in PVW, inc...
Fig 5A: In a vab-3(ot1243) mutant allele, many neurons in the anterior ganglion lose their NeuroPAL ...
Fig 6A: In ceh-32(ok343) null mutant animals, OLL, URY but also RIA markers are almost always lost (...
<p>Null <i>unc-18</i> (<i>e81</i>) worms were rescued transgenically with wild-type (Wt) <i>unc-18</...
<p>(A, B) Genomic analysis to confirm that single-copy insertion has occurred. (A) Scheme showing th...
International audienceMultiple advances have been made to increase the efficiency of CRISPR/Cas9 edi...
Representative fluorescence images of wild-type, asna-1(ok938), asna-1(ΔHis164) and asna-1(A63V) adu...
<p>(<b>A</b>) Representative western blots showing the expressed full-length Tau (0N4R and 0N3R) and...
<p>A. Micrographs of fixed young adult hermaphrodites stained with Oil Red O (top panels) or Sudan B...
A) C. elegans dye uptake assay. In wild type worms, DiI dye is taken up by 6 pairs of amphid (head) ...
<p>(A) <i>Left panel</i>: Single worm duplex PCR was performed on ten animals of the <i>fahd-1(tm500...