<i>unc-39</i> controls differentiation of the AIA interneuron class.

unc-39R203Q mutant animals (either canonical e257 allele or CRISPR/Cas9 genome engineered ot1173 allele with identical nucleotide change) were analyzed. Fig 2A: unc-39 affects the cholinergic identity of the AIA interneuron class (unc-17 reporter allele syb4491 and a cho-1 promoter fragment which is part of the otIs653 array), and other AIA terminal identity markers: reporter alleles dmsr-2(syb4514), ins-1(syb5452) and flp-19(syb3278), and a mgl-1 promoter fragment otIs327. We did not quantify changes in AIA in the NeuroPAL color code, because it is variable in wild type. Representative images of wild type and mutant worms are shown with 10 μm scale bars. Graphs compare expression in wild type and mutant worms with the number of neurons examined listed at the bottom of the bar. P-values were calculated by Fisher’s exact test. Fig 2B: unc-39 affects the expression of the tagged eya-1 locus (nIs352 transgene) in AIA.