Fitting simulations to the experimental data.

This figure compares the simulated results (red shades) with experimental data (blue shades) for one data set. The figure has been separated into three regions showing results from the theoretical model, type 1 simulations, and type 3 simulations. Panels (a)-(e), (j), and (k) show all the time data together, whereas the rest show the time data in individual panels. When optimizing only the data in the unshaded area is used. The minimum value of the objective function is 0.0511, 0.0525, and 0.0139 for type 1 and for type 3 0.0415, 0.0404, and 0.0131 for uniform, Gaussian, and gamma distributed velocity respectively. The y axis is fluorescence intensity (a.u.) in all panels. Length: All—uniform with mean 5.025 microns.