Lymphocystis disease virus (LCDV) is responsible for a chronic self-limiting disease that affects more than 125 teleosts. Viral isolation of LCDV is difficult, time-consuming and often ineffective; the development of a rapid and specific tool to detect and quantify LCDV is desirable for both diagnosis and pathogenic studies. In this study, a quantitative real-time PCR (qPCR) assay was developed using a Sybr-Green-based assay targeting a highly conserved region of the MCP gene. Primers were designed on a multiple alignment that included all known LCDV genotypes. The viral DNA segment was cloned within a plasmid to generate a standard curve. The limit of detection was as low as 2.6 DNA copies/mu l of plasmid and the qPCR was able to detect vi...
Quantitative PCR (QPCR, or real time PCR (rtPCR)) has emerged as a powerful virologic technique for ...
In previous research, a 32 kDa protein in lymphocystis disease virus (LCDV) was identified as viral ...
The lymphocystis disease (LCD), the main viral pathology described in cultured gilthead seabream (Sp...
Lymphocystis disease virus (LCDV) is responsible for a chronic self-limiting disease that affects mo...
Lymphocystis disease virus (LCDV), (Lymphocystivirus, Iridoviridae) is the causative agent of lympho...
Lymphocystis is a cutaneous viral disease, caused by a virus (LCDV) of genus Lymphocystivirus, famil...
AbstractLymphocystis disease virus (LCDV) is the causative agent of lymphocystis disease, which has ...
An immunoblot technique for the detection of lymphocystis disease virus (LCDV) in naturally infected...
Lymphocystis disease is a prevalent, non-fatal disease that affects many teleost fish and is caused ...
The orange clownfish, Amphiprion percula, is a popular fish in the global marine ornamental trade. I...
Three serological techniques (indirect immunofluorescence test, flow cytometry, and indirect dot–blo...
Lymphocystis disease virus (LCDV) is the etiological agent of lymphocystis disease (LCD), a patholog...
Lymphocystis disease (LCD) is the main viral infection reported to affect cultured gilthead seabream...
Lates calcarifer herpes virus (LCHV) is a novel virus of farmed barramundi in Southeast Asia. Howeve...
The 27.8 kDa membrane protein expressed in flounder (Paralichthys olivaceus) gill cells was proved t...
Quantitative PCR (QPCR, or real time PCR (rtPCR)) has emerged as a powerful virologic technique for ...
In previous research, a 32 kDa protein in lymphocystis disease virus (LCDV) was identified as viral ...
The lymphocystis disease (LCD), the main viral pathology described in cultured gilthead seabream (Sp...
Lymphocystis disease virus (LCDV) is responsible for a chronic self-limiting disease that affects mo...
Lymphocystis disease virus (LCDV), (Lymphocystivirus, Iridoviridae) is the causative agent of lympho...
Lymphocystis is a cutaneous viral disease, caused by a virus (LCDV) of genus Lymphocystivirus, famil...
AbstractLymphocystis disease virus (LCDV) is the causative agent of lymphocystis disease, which has ...
An immunoblot technique for the detection of lymphocystis disease virus (LCDV) in naturally infected...
Lymphocystis disease is a prevalent, non-fatal disease that affects many teleost fish and is caused ...
The orange clownfish, Amphiprion percula, is a popular fish in the global marine ornamental trade. I...
Three serological techniques (indirect immunofluorescence test, flow cytometry, and indirect dot–blo...
Lymphocystis disease virus (LCDV) is the etiological agent of lymphocystis disease (LCD), a patholog...
Lymphocystis disease (LCD) is the main viral infection reported to affect cultured gilthead seabream...
Lates calcarifer herpes virus (LCHV) is a novel virus of farmed barramundi in Southeast Asia. Howeve...
The 27.8 kDa membrane protein expressed in flounder (Paralichthys olivaceus) gill cells was proved t...
Quantitative PCR (QPCR, or real time PCR (rtPCR)) has emerged as a powerful virologic technique for ...
In previous research, a 32 kDa protein in lymphocystis disease virus (LCDV) was identified as viral ...
The lymphocystis disease (LCD), the main viral pathology described in cultured gilthead seabream (Sp...