Body weight, circulating methylmalonic acid and animal survival in MMA mice on diets with standard (21%) and low (12%) protein content.

Nursing dams were initially on a 21% protein diet. On PND 14, litters were either maintained on the 21% protein diet or switched to a 12% protein diet. (A) Body weight of MMA mice (-/-) and their heterozygous littermates (+/-) on PND 35. All heterozygous groups were significantly heavier than the gender- and diet-matched MMA groups. (B) Circulating methylmalonic acid level in MMA mice at PND 30. (C) Kaplan-Meier survival curves of MMA mice demonstrated significantly better survival of the 12% diet group than the 21% diet group. *** P 0.001, **** P 0.0001, Student’s t-test in (A) and (B). P 0.0001, log-rank test in (C).