Additional file 3 of LncRNA MRF drives the regulatory function on monocyte recruitment and polarization through HNRNPD-MCP1 axis in mesenchymal stem cells

Additional file 3: Fig. S2. MCP1 expression in MSCs during coculture with monocytes. (A-C) Dynamic changes of MCP1 expression in MSCs at different time points during coculture, as measured by qRT–PCR, western blotting and ELISA. (D-F) The knockdown efficiency of MCP1 in monocytes was verified via qRT–PCR, western blotting and ELISA. (G–I) Dynamic changes of MCP1 expression in MSCs at different time points during coculture upon knockdown of MCP1 in monocytes, as detected by qRT–PCR, western blotting and ELISA. Quantitative results of western blotting were normalized to the result for GAPDH. The data are presented as the mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001, ns= not significant. n=3, all experiments were independently repeated three times