Quantifying engagement in specific behaviours.

Ethogram showing percentage of time infected wild-type males spend (A) engaging in micromovements (e.g. feeding and grooming), (B) walking and (C) sleeping in 30-min bins. Boxplots show the quantification of (D) total distance covered when flies were scored as awake and (E) the total number of times that the flies crossed the middle of the housing tube as a proportion of the time (in seconds) spent awake. Uninfected and mock controls are represented by grey and black tracings, respectively. Infected flies are in blue. Distance covered normalized to time awake (Kruskal-Wallis chi-square = 6.496, df = 2, n = 419, p = 0.039; Dunn’s post hoc: mock|F. novicida = 0.033, mock |uninfected = 0.163, uninfected|F. novicida = 0.460) and midline crosses normalized to time awake (Kruskal-Wallis chi-square = 5.453, df = 2, n = 419, p = 0.065; Dunn’s post hoc: mock|F. novicida = 0.064, mock |uninfected = 0.330, uninfected|F. novicida = 0.339) were not impacted by the infection. (PDF)