Pan-neuronal knock-down of the adipokinetic hormone receptor (AKH-R) inhibits the increase of activity induced by starvation.

Ethogram showing percentage of time (A) AkhR-IR>+, (B) n-sybG4>+, and (C) n-sybG4/UAS—AKH-R RNAi male flies spend moving over time in 30-min bins. The grey area indicates the starvation period, when flies were transferred from standard food to starvation food (2% agarose in PBS). Boxplots indicate the quantification of the entire starvation period (Kruskal-Wallis chi-square = 39.088, df = 5, n = 231, p = 2.28e-07; Dunn’s post hoc: UAS—AKH-R RNAi>+ FED|STARVED = 2.33e-05, n-sybG4>+ FED|STARVED = 2.80e-02, n-syb>AKH-R RNAi = 0.480). All genotypes were assayed at the same time but are here shown separately for clarity. Experiments conducted at 25°C. (PDF)