Localisation of VIVβ8 by immunolabelled transmission electron microscopy.
- Alessandra Romero-Ramirez (4582990)
- Aitor Casas-Sánchez (3689866)
- Delphine Autheman (416290)
- Craig W. Duffy (9853941)
- Cordelia Brandt (125287)
- Simon Clare (125268)
- Katherine Harcourt (488197)
- Marcos Rogério André (4348135)
- Kayo José Garcia de Almeida Castilho Neto (13840411)
- Marta M. G. Teixeira (8969402)
- Rosangela Zacharias Machado (13840414)
- Janine Coombes (13840417)
- Robin J. Flynn (3325788)
- Gavin J. Wright (7443272)
- Andrew P. Jackson (7579631)
Publication date
September 2022
DOI Publisher
Public Library of Science (PLoS)Abstract
Parasites (P) and erythrocytes (E) derived from murine infections were labelled with anti-VIVβ8 polyclonal antibodies. VIVβ8 was localised to the whole parasite cell surface (top and bottom left), and to the parasite flagellum (PF). Anti-VIVβ8-coated gold particles are indicated by white arrows. The right-hand image shows the predominant localisation of VIVβ8 to the surface, although intracellular positions are observed. The graph (inset) shows the proportion of labelled cells and a frequency distribution of the proportion of anti-VIVβ8-coated gold particles found adjacent to the parasite cell membrane of labelled cells.</p
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