RBM46 sequence-specific binding to a specific cohort of mRNAs encoding factors required for RNA processing and meiosis.

(A) FLAG-stained western blot of lysates from Rbm46WT/WT and Rbm46FLAG/FLAG showing input and immunoprecipitated proteins, respectively. Band at the expected protein size is indicated by arrow. (B) Genomic distribution of RBM46 binding in the testis, displayed as percentages of binding to each sequence feature. (C) Representative UCSC genome browser view showing exonic binding of RBM46 across Dazl and Meioc. (D) WebLogo showing U-rich RNA binding sequence motif identified using mCross as the most enriched sequence at the RBM46 crosslink sites. (E) Gene ontology analysis performed using EnrichR showing the top biological processes of RBM46-bound mRNAs.