Additional file 4 of Malignant clonal evolution drives multiple myeloma cellular ecological diversity and microenvironment reprogramming

Additional file 4: Supplementary Fig. 4. Cellular heterogeneity in the myeloma of MM patients. (A) Heterogeneity score of the myeloma in NDMM and RRMM patients. (B) Single-cell atlas based on t-SNE showing heterogeneity of malignant subclonal ecological components in different MM patients. (C) Specific markers of malignant subclones in patients with NDMM and RRMM. Left: Expression pattern of malignant subclonal-specific marker genes in patients. Right: TFs that regulate malignant subclonal-specific markers. (D) Contribution of cell subsets with malignant subclone-specific markers in patients. Left: Expression patterns of malignant subclonal-specific marker genes in patients. The malignant subclonal marker genes illustrated are those that are the most significant and associated with subsequent MM malignant plasma cell clonal evolution reprogramming of immune cells in the microenvironment. Right: Contribution of cell subsets specifically labeled by the top specific marker malignant subclones in patients. (E) The single-cell atlas based on t-SNE showing the heterogeneity of cell ecological components in the myeloma microenvironment in different MM patients. TF, transcription factor