Additional file 5 of Malignant clonal evolution drives multiple myeloma cellular ecological diversity and microenvironment reprogramming

Additional file 5: Supplementary Fig. 5. Expression patterns of immune cell subpopulations in MM. (A-C) Expression patterns of known cell identity-specific markers and their co-location with malignant markers in lymphocytes provide evidence for MM reprogramming of microenvironment cells. The pie chart shows the co-location between known cell identity-specific markers and corresponding patient heterogeneous malignant marker immunoglobulins. (D) HSC subset profiles of control donor BM and the MM patient BM microenvironment. Left: Single-cell subpopulation atlas of HSCs. Middle: Proportion of HSCs in control donors and different MM patients. Right: Expression patterns of cell subset-specific markers mapped on the HSC single-cell atlas. (E, F) MM malignant clonal evolution reprograms pseudotime trajectories and expression pattern changes in lymphoid (E) and myeloid cells (F). Lymphocytes include B cells, plasma, CTLs, T cells, and NK cells, whereas myeloid cells include cDCs and monocytes. For each cell type, the pseudotime trajectory (left), pseudotime value change (middle), and expression pattern change (right) from normal cells to MM during MM malignant clonal evolution reprogramming are shown for lymphocyte and myeloid cell subsets. (G) Expression patterns of the exosome-specific marker gene CD63 in malignant cells. HSCs, hematopoietic stem cells; CTLs, cytotoxic T lymphocytes; NK cells, natural killer cells; cDCs, conventional dendritic cells