Using a chemical approach to crosslink functionally versatile bioeffectors (such as peptides) to native proteins of interest (POI) directly inside a living cell is a useful toolbox for chemical biologists. However, this goal has not been reached due to unsatisfactory chemoselectivity, regioselectivity, and protein selectivity in protein labeling within living cells. Herein, we report the proof of concept of a cytocompatible and highly selective photolabeling strategy using a tryptophan-specific Ru-TAP complex as a photocrosslinker. Aside from the high selectivity, the photolabeling is blue light-driven by a photoinduced electron transfer (PeT) and allows the bioeffector to bear an additional UV-responsive unit. The two different photosensit...
Genetically encodable optical reporters, such as green fluorescent protein, have revolutionized the ...
Biomedical research has been empowered by tools that enable spatial and temporal control of biologic...
Optical modulation of proteins provides superior spatiotemporal resolution for understanding biologi...
An ability to manipulate the activity of a specific protein inside living cells offers exciting pros...
Many biological processes are governed by protein activities that are spatially and temporally regul...
Genetically-encodable optical reporters, such as Green Fluorescent Protein, have revolutionized the ...
Summary. Nature employs very sophisticated strategies of using light to control certain biological p...
Optogenetics enables the spatio-temporally precise control of cell and animal behavior. Many optogen...
In recent years, interest in controlling protein function with light has increased. Light offers a n...
Controlled manipulation of proteins and their function is important in almost all biological discipl...
Photochemical transformations enable exquisite spatiotemporal control over biochemical processes; ho...
Proteins and their complex dynamic interactions regulate cellular mechanisms from sensing and transd...
A growing number of optogenetic tools have been developed to reversibly control binding between two ...
Living materials are based on proteins that adapt and change in structure and function continuously ...
Photoswitchable ligands are powerful tools to control biological processes at high spatial and tempo...
Genetically encodable optical reporters, such as green fluorescent protein, have revolutionized the ...
Biomedical research has been empowered by tools that enable spatial and temporal control of biologic...
Optical modulation of proteins provides superior spatiotemporal resolution for understanding biologi...
An ability to manipulate the activity of a specific protein inside living cells offers exciting pros...
Many biological processes are governed by protein activities that are spatially and temporally regul...
Genetically-encodable optical reporters, such as Green Fluorescent Protein, have revolutionized the ...
Summary. Nature employs very sophisticated strategies of using light to control certain biological p...
Optogenetics enables the spatio-temporally precise control of cell and animal behavior. Many optogen...
In recent years, interest in controlling protein function with light has increased. Light offers a n...
Controlled manipulation of proteins and their function is important in almost all biological discipl...
Photochemical transformations enable exquisite spatiotemporal control over biochemical processes; ho...
Proteins and their complex dynamic interactions regulate cellular mechanisms from sensing and transd...
A growing number of optogenetic tools have been developed to reversibly control binding between two ...
Living materials are based on proteins that adapt and change in structure and function continuously ...
Photoswitchable ligands are powerful tools to control biological processes at high spatial and tempo...
Genetically encodable optical reporters, such as green fluorescent protein, have revolutionized the ...
Biomedical research has been empowered by tools that enable spatial and temporal control of biologic...
Optical modulation of proteins provides superior spatiotemporal resolution for understanding biologi...