Chimeric SARS-CoV-2-Khosta 2 spike is resistant to current vaccines.

(A) The RBD from SARS-Cov-2 spike was replaced with Khosta 2 RBD. (B) Pseudotpyes with indicated chimeric spikes were used to infect 293T cells stably expressing human ACE2. Pseudotypes were combined with (C) bamlanivimab or (D) vaccinated patient serum at various concentrations and used to infect 293T-hACE2 cells.(E) Area under the curve analysis for data in panel D. p-value 0.0021 (**), 0.0002 (***), F) Pseudotypes with Khosta 2 or Omicron variant RBD were combined with serum from vaccinated patients with breakthrough Omicron infection and used to infect 293T-hACE2 cells. (G) Area under the curve analysis for data in panel F. p-value 0.0021 (**), 0.0002 (***), H) Sequence identity matrix for RBD amino acid sequences from Khosta 2 and known SARS-CoV-2 variants of concern.