RNAi silencing of EcR results in elevated activation of PGRP-LC and Rel2 in response to bacterial infection.

(A and B) Immunofluorescence of PGRP-LC (A) and Rel2 (B) in iEGFP and iEcR mosquito fat bodies, stimulated for 12 h with PBS or E. cloacae 12 h after blood meal. Scale bar, 20 μm. (C and D) Quantification of relative fluorescence intensity of cells stained using PGRP-LC or Rel2 antibodies. (E) Protein levels of Rel2 in the nucleus of iEGFP and iEcR mosquitoes that were either non-infected or infected with E. cloacae. For each group, nuclear protein extracts were performed from extracts of fat bodies from ten female mosquitoes after 24 h of E. cloacae challenge. Histone H3 were used as controls. Data are from three biological replicates.