ITGB1 in THP-1 and CAV-1 in HUVEC responsible for internalization of leptospires.

A: Distribution of ITGB1, ITGB2, ITGB3 and CAV-1 in THP-1 and HUVEC determined by flow cytometry. Ten thousand cells were analyzed for each of the specimens; B: Leptospires in THP-1 and HUVEC under the laser confocal microscope after infection with L. interrogans strain Lai for 1 h. The blue plaques in the middle of cells indicate the nuclus. The red spots around the nuclus indicate the intracellular leptospires; C: Statistical summary of red fluorescence intensity reflecting the leptospires in THP-1 and HUVEC after infection with L. interrogans strain Lai for 1 h. Statistical data from experiments such as shown in B. Bars show the means ± SD of three independent experiments. One hundred cells were analyzed for each of the specimens. *: p vs the red fluorescence intensity reflecting the leptospires in the wild type cells during infection. D: Relative gene expression of ITGB1, ITGB2 ITGB3, CAV-1 in THP-1 or HUVEC were analysed by comparing treatment of 4h of leptospires infection and non-infection. Bars show the means ± SD of three independent experiments. *: p vs the gene relative expression of treatment of non-infection.