Overexpression of AFF4 enhances adipogenesis.

(A-D) Adipogenesis in AFF4-overexpressing cells. (A) Representative images of oil red o staining of 3T3-L1 cells. (B) Quantification of oil red o staining, absorbance at 518 nm. n = 3. (C) Triglyceride quantification. n = 3. (D) RT-qPCR of adipogenic markers. n = 3. (E-H) Ectopic fat formation in nude mice. (E) Xenograft of oeScram and oeAFF4 3T3-L1 cells in nude mice and samples were collected after 6 weeks. Scale bar, 1mm. (F) Fat pad size quantification. n = 6. (G) Representative immunofluorescent images of lipids. Scale bar, 50 μm. (H) Adipocyte size quantification. n = 6. All quantified data are presented by as mean ± SD. The P values were calculated by two-tailed Student’s t-test.