Additional file 5 of ATP-citrate lyase B (ACLB) negatively affects cell death and resistance to Verticillium wilt

Additional file 5: Fig. S5. Validation of Arabidopsis (AT5G49460, aclb-2) mutants. (a) Schematic diagram showing two pairs of primers to PCR for validating T-DNA insertion. (b) The results of PCR amplification of Col-0 and aclb-2 mutants. (c) The relative expression level of AtACLB-2 in Col-0 and aclb-2. Data are shown as mean ± SE of three biological replicates (**P < 0.01, Student’s t-test). (d) The 14-days-old seedlings of the Col-0 grew obviously faster than that of aclb-2. Scale bars, 1 cm. (e) Root of Col-0 was obviously longer than that of aclb-2. Data are shown as mean ± SE of six biological replicates (**P < 0.01, Student’s t-test)