Additional file 1 of Development of a 64Cu-labeled CD4+ T cell targeting PET tracer: evaluation of CD4 specificity and its potential use in collagen-induced arthritis

Additional file 1: Figure S1 Full-length SDS-PAGE. (a) Coomassie staining of a full-length SDS-PAGE with ladder. (b) radiography of the same full-length stained SDS-PAGE. Figure S2 Gating strategy used to identify CD4+ T cells and neutrophils by flow cytometry. Percent of (a) CD45+ T cells and (b) viable cells in the carpal and tarsal joints from control, DXM-treated and untreated CIA mice. Mice with CIA were scored according to degree of inflammation (swelling and erythema) in each paw on a scale from 0–4. Each point represents a joint. Data are pooled from two independent experiments. The significance level is indicated by asterisks (*). p = 0.02–0.04 (*) and p < 0.0001 (****). (c) Both populations were gated based on time, as singlets, based on scatter profile, lack of staining by viability dye and as positive for CD45. CD4+ T cells were further defined as CD3+ CD4+. Neutrophils were further defined as CD11b+ Ly6g+