Esp452 inhibited within intact N-terminal region.

A. Luminescence of biofilm fractions of MMH594b (Δesp) biofilms grown with PBS, Esp743, or Esp452, divided by the average luminescence of biofilm fractions from biofilms grown with PBS. The experiment was conducted with triplicates in three independent experiments. The SEM is indicated with error bars. Samples were compared by Welch’s ANOVA and D3 Dunnett’s post hoc test. **** p B. Fluorescence of Thioflavin T added to MMH594b (Δesp) biofilms that were grown with Esp452, Esp453-743, or Esp743. Fluorescence measurements from three independent experiments were combined. The SEM is indicated with error bars. The 95% CI for the dose-response curve of Esp452 is shown as dashed lines. C. Confocal microscopy images of MMH594b (Δesp) biofilms grown with PBS, Esp743-AF647, or Esp452-AF647 (red). Biofilms were stained with Syto-13 (green). The images represent Z-stacks with the greatest number of red pixels.