IL-23 is required for WD-induced hepatic IL-17 producing cell expansion.

WT or Il23r-/- mice were fed a ND or WD for 20 weeks, followed by terminal analyses (A). FACS quantification of percent RORγt+ from γδ T Cells and RORγt+ from MAIT cells (CD3e+TCRb+CD4-CD8-) (B-C). Percentage of IL-17A positive cells in γδ T cells from WD fed WT and Il23r-/- liver NPCs stimulated with leukocyte activation cocktail with GolgiPlug (BD) for 4 hours (D). Groups: ND WT n = 5, ND Il23r-/- n = 5, WD WT n = 7, WD Il23r-/- n = 7. Data represents mean ± S.D. *p < 0.05, **p < 0.005, one-way ANOVA.