A) Unstimulated HUVEC displayed no activity after incubation with 1μM N/OFQ. B) Stimulation of ERK1/2 in LPS/PepG treated HUVEC by 1μM N/OFQ was measured over 30 minutes. Representative Western blots are shown below the graph. Data are the mean±SEM from 20 separately cultured lines. Analysis was undertaken on raw data using ANOVA and Bonferroni post hoc analysis, *statistically greater than raw basal activity (p<0.05).</p
<p>Western blot detection of p-ERK1/2 and ERK1/2 proteins in proliferating (A) and non-proliferating...
<p>Fibroblasts were stimulated for 2% CSE or 1, 5, 10 or 20% BME in 0.1% FBS/DMEM, before whole cell...
<p>(A–C) The cells were maintained in 5% DCC for 48 hours and then treated with E2 (10 nM), DPN (10 ...
In the HUVEC line PN5 that expresses NOP receptors in the absence of LPS/PepG treatment, addition of...
<p><b>A.</b> and <b>B.</b> Bar graphs show the time-dependent changes in the phosphorylation status ...
<p>Time-course of ERK1/2 and NFκB phosphorylation after 10% or 20% PL treatment of HUVECs. Data are ...
<p>(A) Western blot analysis of phospho-Erk1/2 MAPKs in wt or p130Tg organoids stimulated with EGF a...
<p>A) NIH 3T3 cells have been starved for 24 hr before treatment for 15 min with increasing concentr...
Cells were pretreated with the PKC inhibitor, Gö6983 (a), or the EGFR Tyr kinase inhibitor, AG1478 (...
<p>(A) Effects of U-II on phosphorylated of Akt and ERK. HUVECs were incubated with 15 min with U-II...
<p>The upper side of panel (A) shows a representative Western blot for Phospho-p44/42 MAPK (Erk1/2) ...
<p><b>a </b><i>Left panel</i>, relative levels of phosphorylated ERK1/2 total ERK1/2 in ERoSHK<sub>L...
Figure 2: Kinase presence and activity in TNF-α-stimulated HUVEC. The influence of different dura...
<p><b>a.</b> Comparison of phospho-ERK1/2 activation in hLH-stimulated <i>vs</i> unstimulated (contr...
<p>HPAECs were grown to confluence in 6-cm dishes, starved for 16 hrs and then stimulated with BMP-9...
<p>Western blot detection of p-ERK1/2 and ERK1/2 proteins in proliferating (A) and non-proliferating...
<p>Fibroblasts were stimulated for 2% CSE or 1, 5, 10 or 20% BME in 0.1% FBS/DMEM, before whole cell...
<p>(A–C) The cells were maintained in 5% DCC for 48 hours and then treated with E2 (10 nM), DPN (10 ...
In the HUVEC line PN5 that expresses NOP receptors in the absence of LPS/PepG treatment, addition of...
<p><b>A.</b> and <b>B.</b> Bar graphs show the time-dependent changes in the phosphorylation status ...
<p>Time-course of ERK1/2 and NFκB phosphorylation after 10% or 20% PL treatment of HUVECs. Data are ...
<p>(A) Western blot analysis of phospho-Erk1/2 MAPKs in wt or p130Tg organoids stimulated with EGF a...
<p>A) NIH 3T3 cells have been starved for 24 hr before treatment for 15 min with increasing concentr...
Cells were pretreated with the PKC inhibitor, Gö6983 (a), or the EGFR Tyr kinase inhibitor, AG1478 (...
<p>(A) Effects of U-II on phosphorylated of Akt and ERK. HUVECs were incubated with 15 min with U-II...
<p>The upper side of panel (A) shows a representative Western blot for Phospho-p44/42 MAPK (Erk1/2) ...
<p><b>a </b><i>Left panel</i>, relative levels of phosphorylated ERK1/2 total ERK1/2 in ERoSHK<sub>L...
Figure 2: Kinase presence and activity in TNF-α-stimulated HUVEC. The influence of different dura...
<p><b>a.</b> Comparison of phospho-ERK1/2 activation in hLH-stimulated <i>vs</i> unstimulated (contr...
<p>HPAECs were grown to confluence in 6-cm dishes, starved for 16 hrs and then stimulated with BMP-9...
<p>Western blot detection of p-ERK1/2 and ERK1/2 proteins in proliferating (A) and non-proliferating...
<p>Fibroblasts were stimulated for 2% CSE or 1, 5, 10 or 20% BME in 0.1% FBS/DMEM, before whole cell...
<p>(A–C) The cells were maintained in 5% DCC for 48 hours and then treated with E2 (10 nM), DPN (10 ...