(A) HPIV3 infection caused VIM to aggregate. HeLa cells were infected with HPIV3 for 24 h (MOI = 0.1), goat anti-HPIV3 and mouse anti-VIM were used; scale bar: 10 μm. (B) HPIV3 IBs co-located with VIM and microtubules, and VIM suppressed the fusion of HPIV3 IBs. HeLa-GFP–P cells infected with HPIV3 (MOI = 0.1) and transfected with mCherry–VIM plasmid and microtubules were labeled with paclitaxel. Scale bar: 2 μm (S2 Movie and S3 Movie). The white arrow shows the fusion of IBs, and the black arrow shows the IBs with VIM and microtubules. (C) IB fusion time statistics corresponding to (B). Individual data represent the IB contact and fusion times. Numbers of IBs adjacent to microtubules or to both mCherry–VIM and microtubule. The GFP–P indica...
<p>(<b>A</b>) Western blot analysis of cell vimentin in the soluble (top panels) and insoluble (bott...
<p>(A, B) Representative confocal images of intracellular localization of G3BP1, TIA1 and HuR follow...
(A) Interaction between VIM mutants and the N–P complex. VIM head domain-related mutant (VIMΔ20, VIM...
(A) VIM downregulated α-tubulin acetylation. N-Myc, HA–P, and Flag–VIM plasmids were transfected alo...
(A) α-TAT1 interacted with VIM but not with VIMΔH. Myc–α-TAT1 plasmid was co-transfected with Flag–V...
(A) VIM did not impair the binding and entry of HPIV3. Schematic diagram of HPIV3 binding and entry ...
(A) Overexpression of VIM suppressed HPIV3 infection. Flag–VIM plasmid was transfected into HeLa and...
(A) Experimental design flow chart. HPIV3 N–P complex expressed in HEK293T cells was immunoprecipita...
<p>(A and B) HeLa cells were co-transfected with plasmids encoding GFP-P and N-Myc, GFP-P and NΔN10-...
Model of VIM’s inhibitory effect on the fusion of HPIV3 IBs and viral replication.</p
This movie is as shown in Fig 3E. The mCherry–VIM plasmid was transfected into HeLa-GFP–P cells, and...
<p>(A) HeLa cells were transfected with plasmids encoding GFP-P and N-Myc for 24 h, then transfected...
AbstractVif forms a complex with Elongin B/C, Cullin-5 and Rbx-1 to induce the polyubiquitination an...
<p>(A) Analysis of LC3 modification following infection with USUV Vienna 2001. Vero cells infected o...
A) Immunofluorescence analysis of endogenous vimentin (white), tubulin (red) and GFP-LC3 in HEK293-G...
<p>(<b>A</b>) Western blot analysis of cell vimentin in the soluble (top panels) and insoluble (bott...
<p>(A, B) Representative confocal images of intracellular localization of G3BP1, TIA1 and HuR follow...
(A) Interaction between VIM mutants and the N–P complex. VIM head domain-related mutant (VIMΔ20, VIM...
(A) VIM downregulated α-tubulin acetylation. N-Myc, HA–P, and Flag–VIM plasmids were transfected alo...
(A) α-TAT1 interacted with VIM but not with VIMΔH. Myc–α-TAT1 plasmid was co-transfected with Flag–V...
(A) VIM did not impair the binding and entry of HPIV3. Schematic diagram of HPIV3 binding and entry ...
(A) Overexpression of VIM suppressed HPIV3 infection. Flag–VIM plasmid was transfected into HeLa and...
(A) Experimental design flow chart. HPIV3 N–P complex expressed in HEK293T cells was immunoprecipita...
<p>(A and B) HeLa cells were co-transfected with plasmids encoding GFP-P and N-Myc, GFP-P and NΔN10-...
Model of VIM’s inhibitory effect on the fusion of HPIV3 IBs and viral replication.</p
This movie is as shown in Fig 3E. The mCherry–VIM plasmid was transfected into HeLa-GFP–P cells, and...
<p>(A) HeLa cells were transfected with plasmids encoding GFP-P and N-Myc for 24 h, then transfected...
AbstractVif forms a complex with Elongin B/C, Cullin-5 and Rbx-1 to induce the polyubiquitination an...
<p>(A) Analysis of LC3 modification following infection with USUV Vienna 2001. Vero cells infected o...
A) Immunofluorescence analysis of endogenous vimentin (white), tubulin (red) and GFP-LC3 in HEK293-G...
<p>(<b>A</b>) Western blot analysis of cell vimentin in the soluble (top panels) and insoluble (bott...
<p>(A, B) Representative confocal images of intracellular localization of G3BP1, TIA1 and HuR follow...
(A) Interaction between VIM mutants and the N–P complex. VIM head domain-related mutant (VIMΔ20, VIM...