Antibody responses of the <i>P</i>. <i>falciparum-</i>infected patients to PfGCS1 antigen.

A) anti-PfGCS1 IgG antibody responses to rPfGCS1 antigen among plasma samples obtained from Iranian P. falciparum-infected patients (n = 171) were measured using ELISA. Fifteen plasma samples from non-exposed individuals were used as negative controls. The dots represent the OD450nm for each plasma sample, and the stars and vertical lines indicate the mean ODs and standard deviations (SD) for each group, respectively. The cut-off for the anti-rPfGCS1 IgG antibody was 0.51 (mean of negative controls + 3SD). B) IgG antibody responses against PfGCS1-SAPN and NC-SAPN among PfGCS1 seropositive plasma samples (n = 30), PfGCS1 seronegative plasma samples (n = 30), and non-exposed individuals (n = 15). The dots represent the OD450nm for each plasma sample, and the stars and vertical lines indicate the mean ODs and standard deviations (SD) for each group, respectively. The cut-off for the anti-PfGCS1-SAPN IgG antibody was 0.585 (mean of negative controls + 3SD).