Hepatic LPL overexpression maintains mitochondrial content in HFD-fed mice.

Male C57BL/6 mice were intravenously treated with Ad vectors via the tail vein and were simultaneously fed HFD. PBS-treated mice were fed ND throughout the experimental period. Two weeks after administration of Ad-LPL, Ad-Luc, or PBS, (A, C) expression of mitochondrial genes in the liver determined using quantitative RT-PCR. The data were normalized to the corresponding values in the Ad-Luc-treated group. One-way ANOVA with Dunnett’s post hoc tests was used for multiple comparisons. Hepatic (B) citrate synthase protein levels in male C57BL/6 mice 2 weeks after administration of Ad-LPL or Ad-Luc determined using western blot analysis. The quantification data were normalized to the corresponding value in the Ad-Luc-treated group. The Mann–Whitney U test was used to compare the differences between two independent groups. (D) The area of mitochondria in the liver was assessed via transmission electron microscopy analysis. Arrowheads indicate mitochondria. Bar = 5 μm. (E) The area of mitochondria was determined by measuring the area of mitochondria in the cell using Fig 4D. The Mann–Whitney U test was used to compare the differences between two independent groups. The data are expressed as the mean ± standard error (A–C: n = 5, E: n = 151–155). *, P < 0.05 vs. Ad-Luc.