Additional file 2 of Sperm DNA methylation alterations from cannabis extract exposure are evident in offspring

Additional file 2: Figure S2. Pyrosequencing validation of WGBS CpG sites. Bisulfite pyrosequencing of CpG sites initially identified via WGBS as significantly differentially methylated between late exposed sperm and controls for A Hoxb9; B Mettl11b; C Sycp3; D Cit; E Slit2; F Lrp1; G Cit; and early exposed and controls H Grin2a; I Syn3. CpG sites are identified as A-D on the x-axis, and the recorded methylation level is on the y-axis. Each point is an individual sperm. Black = controls, green = late exposed, blue = early exposed. *p < 0.05