Sample experiment timeline.

This timeline represents a short segment of an experiment, illustrating the order and timing of events. Stimuli were injected into a port in the perfusion line (colored dots), traveled down the perfusion line for several seconds, then washed across the olfactory epithelium (“stimulus exposure” period, marked on timeline). EOG responses were observed during this exposure time if evoked by the stimulus. Positive controls (shown in red; amino acids such as methionine and alanine) were used to elicit a strong EOG response at the start and end (not shown) of each recording block. After positive controls or test stimuli, a saline wash was administered to ensure the port and line were clean (white oval). Saline was also used as a negative control (blue). Test stimuli (green) at various dilutions were alternated with negative controls for the bulk of each recording block.