Additional file 1 of TSLP promoting B cell proliferation and polarizing follicular helper T cell as a therapeutic target in IgG4-related disease

Additional file 1: Figure S1. Purity of CD19+ B cells and CD4+ Naïve T cells. FACS file showed CD19+ B cells (A) and CD4+ naïve T cells (B) were sorted to obtain 98% purity. Figure S2. There was no significant difference of TSLP levels between IgG4-RD patients with and without allergic history. Figure S3. Gating strategy and representative FACS plots of TSLPR and IL-7Ra in CD19+ cells of HC and IgG4-RD patients. Figure S4. There was no correlation between plasma TSLP level and other laboratory parameters. Figure S5. TSLP could not polarize Naïve T cells to Tfh directly. Figure S6. The percentage of Tfh was positively correlated with the percentages of OX40L+ on B cells in the peripheral blood of patients with IgG4-RD. Figure S7. Representative western blot showed the phosphorylation of JAK-STAT family with different time. Figure S8. Weight, imaging findings and TSLP expression of Lat mice. Figure S9. Immunohistochemistry of IgG1 in affected organs of Lat mice. Figure S10. Expressions TSLP and TSLPR in affected organs of Lat mice. Figure S11. Application of different dosage of anti-TSLP antibody Lat mice