A comparison of <i>CYP1B1</i> gene conservation among different homologs for novel variants detected in this study.

a) Clustal Omega multiple sequence alignment (MSA) (Shown in red) for p.Arg153Gly detected in PCG049. b) Multiple sequence alignment (MSA) for p. Ser172Arg among homologs detected in PCG049 showing less conservation in Mus musculus. c) MSA result for p.Gly211Argfs*13 among homologs detected in PCG050. d) MSA result for variant p.Val241Glu detected in PCG052. e) MSA result for p.Leu97Ala*127 among homologs detected in PCG053. f) MSA result for p.Arg222Profs*2 detected in PCG054 showing less conservation in Mus musculus. g) MSA result for variant p.Val254Glyfs*73 detected on PCG059 showing complete conservation among homologs. h) MSA result for p. Leu247Gln among homologs detected in PCG060. i) MSA result for p.Phe421Leu detected in PCG062. j) MSA result for p.Leu264Alafs*63 detected in PCG063. k) MSA result for p.Asp242His detected in PCG067. a-k) WebLogo results of all novel CYP1B1 protein variants showing comparison of conservation among homologs are on right side of the figure. All the variants except p.Ser172Arg and p.Arg222Profs*73 show 100% conservation among different mammals (Large size of amino acid abbreviation letter show full conservation while small size show less conserved position among homologs).