DDX39B/DDX39A recruits TREX complex subunits THOC1, THOC4 and CIP29 to form a complex.

(A, B and C) HEK293T cells were co-transfected with MYC-THOC1 (A), HA-THOC4 (B) or MYC-CIP29 (C) and FLAG-DDX39B (WT), FLAG-DDX39B (95A) or empty pCAGGS vectors, at 48h after transfection, the cells were lysed, followed by co-IP with anti-FLAG mouse Mab and western blotting using the antibodies specified in the legend. (D and E) DDX39B-GFP (D) or DDX39A-mCherry (E) together with NP were transfected into HEK293T cells, 24 hours after transfection, the cells were fixed, permeabilized, and stained with anti-NP mouse Mab together with anti-CIP29, anti-THOC1 or anti-THOC4 rabbit PcAb, followed by immunostaining with goat anti-mouse DyLight 405-labeled secondary antibody and goat anti-rabbit FITC-labeled secondary antibody or goat anti-rabbit Alexa Fluor 546-labeled secondary antibody. Images were acquired using confocal microscopy. (TIF)