To detect Tregs, spleens and MLNs were isolated to prepare single cell suspensions for CD4, CD25 and Foxp3 mAbs staining and flow cytometry analysis. (A) Frequency of Treg cells in splenocytes. (B) Frequency of Treg cells in mesenteric lymphocytes. Data are shown as mean ± SEM (n = 4). *P < 0.05.</p
<p>A. Example of analysis of splenocytes from a C57BL/6 mouse transduced with AAV-LacZ and, 6 weeks ...
<p>7 days after sensitization, OVx -OVA and Sham -OVA mice were sacrificed and spleen and lymph node...
<p>(A) Dot plots were gated on CD4<sup>+</sup> T cells (based on forward and side scatter and CD4 st...
<p>Cells were isolated from spleen and MLN on day 7, 14, 21, and 35 following DSS treatment and dete...
<p>(a), Splenocytes were stained with FITC-CD4 and PE-FoxP3 antibody, the frequencies of Treg cells ...
<p>Single-cell suspensions of nucleated thymocytes from intact NIK KO or WT mice were stained with t...
<p>Flow cytometry showing the percentages (A, B) and absolute numbers (C) of CD4<sup>+</sup>CD25<sup...
<p>Typical plots depicting numbers of Tregs in mouse splenocytes (a) and mesenteric lymph node (b) i...
<p>Single cell suspension from spleen, tumor draining lymph nodes, and tumor of control and MIP trea...
<p>Single-cell spleen suspensions from <i>C</i>. <i>sinensis</i>-infected and control mice (NC) were...
<p>SL4-MUC1 cells (1×10<sup>6</sup> cells/50 µL) were injected into the space under the cecal serosa...
<p>Flow cytometry graphs showing the cell populations in spleen from one representative mouse from e...
<p>Representative dot plots are shown for CD4<sup>+</sup>CD25<sup>+</sup>FOXP3<sup>+</sup> cells. Th...
<p>C57BL/6 mice were challenged with E.G7 and then, 7 days post tumor challenge, were treated as ind...
<p>7 days after ovariectomy, OVx and Sham mice were sacrificed and spleen, lymph nodes and thymus ce...
<p>A. Example of analysis of splenocytes from a C57BL/6 mouse transduced with AAV-LacZ and, 6 weeks ...
<p>7 days after sensitization, OVx -OVA and Sham -OVA mice were sacrificed and spleen and lymph node...
<p>(A) Dot plots were gated on CD4<sup>+</sup> T cells (based on forward and side scatter and CD4 st...
<p>Cells were isolated from spleen and MLN on day 7, 14, 21, and 35 following DSS treatment and dete...
<p>(a), Splenocytes were stained with FITC-CD4 and PE-FoxP3 antibody, the frequencies of Treg cells ...
<p>Single-cell suspensions of nucleated thymocytes from intact NIK KO or WT mice were stained with t...
<p>Flow cytometry showing the percentages (A, B) and absolute numbers (C) of CD4<sup>+</sup>CD25<sup...
<p>Typical plots depicting numbers of Tregs in mouse splenocytes (a) and mesenteric lymph node (b) i...
<p>Single cell suspension from spleen, tumor draining lymph nodes, and tumor of control and MIP trea...
<p>Single-cell spleen suspensions from <i>C</i>. <i>sinensis</i>-infected and control mice (NC) were...
<p>SL4-MUC1 cells (1×10<sup>6</sup> cells/50 µL) were injected into the space under the cecal serosa...
<p>Flow cytometry graphs showing the cell populations in spleen from one representative mouse from e...
<p>Representative dot plots are shown for CD4<sup>+</sup>CD25<sup>+</sup>FOXP3<sup>+</sup> cells. Th...
<p>C57BL/6 mice were challenged with E.G7 and then, 7 days post tumor challenge, were treated as ind...
<p>7 days after ovariectomy, OVx and Sham mice were sacrificed and spleen, lymph nodes and thymus ce...
<p>A. Example of analysis of splenocytes from a C57BL/6 mouse transduced with AAV-LacZ and, 6 weeks ...
<p>7 days after sensitization, OVx -OVA and Sham -OVA mice were sacrificed and spleen and lymph node...
<p>(A) Dot plots were gated on CD4<sup>+</sup> T cells (based on forward and side scatter and CD4 st...