Effects of Co colonocytes on nF and mF fibroblasts in coculture system.

nF or mF fibroblasts were seeded into the bottom of 6-well culture plates. Epithelial cells were cultured on a membrane insert for 72 h with nF or mF fibroblasts. A) Effect on the number of nF and mF fibroblasts. nF or mF Fibroblastic cells cultured alone (without Co cells in coculture) were defined as a control. The graphs represent the mean ± SEM values from 4 independent experiments. ***PCM-nF/Co: conditioned medium resulting from the coculture between nF fibroblasts and Co cells; CM-mF/Co: conditioned medium resulting from coculture between mF fibroblasts and Co cells; CM-nF: conditioned medium of nF fibroblasts in monoculture; CM-mF: conditioned medium of mF fibroblasts in monoculture. (TIF)