CVB infection increases in a genetic knockout of <i>TBK1</i>.

Wild-type (TBK1+/+), heterozygous knockout (TBK1+/-), and homozygous knockout (TBK1-/-), mouse embryonic fibroblasts (MEFs) were infected with eGFP-expressing coxsackievirus B at a multiplicity of infection of 10 (MOI 10). (A) Fluorescence microscopy of infected MEFs at 24 h post-infection (p.i.). Phase contrast images show similar cell density at 24 h p.i. Scale bars represent 100 μm. (B) Extracellular viral titers of infected cells at 24 h as measured by plaque assay. ***, p t test n = 3. (C) Diameter of viral plaques. ****, p t test; n = 10. (D) Western blots of infected cells at 24 h p.i. including densitometry. A representative Western blot is shown. **, p t test; n = 3. Data are representative of 3 experiments. WL = whole lysate.