Silencing <i>GABARAPL1</i> and <i>GABARAPL2</i> reduces CVB infection and secretion into EVs.

(A) HeLa cells were treated with siRNA targeting GABARAPL1 and GABARAPL2 (siGABA1/2) and subsequently infected with eGFP-CVB for 24 h. Fluorescence microscopy of HeLa cells infected at 24 h p.i. at MOI 0.01. Phase contrast images show similar cell density at 24 h p.i. Scale bars represent 100 μm. (B) Extracellular viral titers of free virus (free) or EV virus (EV) at 24 h p.i. at MOI 0.1 as measured by plaque assay. ***, p p p p p siSCRAMBLE or siGABA1/2 cells were treated with vehicle or bafilomycin (100 nM) for 2 h. Cell lysates were analyzed by Western blot and densitometry quantification was performed. *, p < 0.05, two-way ANOVA; n = 3. Data are representative of 3 experiments. WL = whole lysate.