Systematic Survey of the Regulatory Networks of the Long Noncoding RNA BANCR in Cervical Cancer Cells br

Long noncoding RNAs (lncRNAs) are increasingly recog-nized as functional regulators of human cancers. BRAF-activated noncodingRNA (BANCR), an oncogenic lncRNA, has a carcinogenic effect on manytypes of cancers. However, the clinical significance and molecularmechanisms of BANCR in cervical cancer are still unclear. Here, wegenerated BANCR knockout cell linesviaCRISPR/Cas9 editing andrevealed that BANCR plays roles in the apoptosis, proliferation, andmigration of HeLa cells. A quantitative proteomics strategy was employed toglobally identify BANCR-regulated proteins in HeLa cells. In total, weidentified 569 differentially expressed proteins (DEPs) upon knockout ofBANCR in HeLa cells. Bioinformatic analysis revealed that these DEPs wereinvolved in diverse cellular pathways. Functional studies revealed thatBANCR exerts its effects on the proliferation and apoptosis of HeLa cellsthrough the regulation of cAMP-responsive element binding protein 1(CREB1) expression. Mechanistically, BANCR could inhibit the expression of miR-582-5p, and CREB1 is a direct target of miR-582-5p; therefore, BANCR may exert its function by regulating CREB1 expressionviatargeting miR-582-5p in cervical cancer cells.Collectively, this study established a proteome-wide BANCR regulatory network, which provides novel insight into the molecularpathogenesis of cervical cancer and can serve as a basis for the development of targeted therapies