Functional redundancy and compensation: Deletion of multiple murine Crisp genes reveals their essential role for male fertility
- Curci, Ludmila
- Brukman, Nicolás Gastón
- Weigel Muñoz, Mariana
- Rojo, Daniela
- Carvajal, Guillermo
- Sulzyk, Valeria
- Gonzalez, Soledad Natalia
- Rubinstein, Marcelo
- Da Ros, Vanina Gabriela
- Cuasnicu, Patricia Sara
DOIAbstract
Mammalian Cysteine-RIch Secretory Protein (CRISP) family includes four members present in sperm and reported to regulate Ca2+ channels and fertilization. Based on our previous observations using single knockouts models and suggesting the existence of functional compensation among CRISP proteins, we investigated their relevance for male fertility by generating multiple Crisp gene mutants by CRISPR/Cas9 technology. Whereas targeting of Crisp1 and Crisp3 yielded subfertile males with early embryo developmental defects, the same deletion in zygotes from fertile Crisp2-/- .Crisp4-/- mice led to the generation of both triple and quadruple knockout mice exhibiting a complete or severe disruption of male fertility due to a combination of sperm tran...
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