Simplified MLST scheme for direct typing of Leptospira human clinical samples
- Varni, Vanina Delia
- Chiani, Yosena
- Nagel, Ariel Gastón
- Ruybal, Paula
- Vanasco, Norma Bibiana
- Caimi, Karina Cynthia
DOIAbstract
Leptospirosis is a globally distributed zoonosis. Epidemiological data are scarce and present major challenge because of the varied clinical presentations. Multilocus Sequence Typing has already proven to be a robust molecular typing method providing accurate results for strain characterization. We have adapted our MLST scheme by reducing the set of loci to facilitate Leptospira typing directly from human clinical samples. The application of this 3-locus scheme provides Leptospira species and allelic profiles of the samples retaining the power of discrimination of the whole scheme. Moreover, an approach to the serogroups was also achieved. Our results contribute to the epidemiological study of Leptospirosis, since the direct typing on clini...
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