Peer Review In Vitro Antioxidant Activity of Lampes (Ocimum sanctum) Leaves and Seeds Ethanol Extract Using DPPH Method

Background. Recent study reported that polyphenol compounds have a large contributionin antioxidant activity. Polyphenol compound was reported as one of the content of Ocimum sanctum.L The aim of this study was to observe the antioxidant activity of lampes (Ocimum sanctum) leaves and seeds ethanol extract using DPPH method. Method. The extraction was done using maceration method. The antioxidant activity was measured byDPPH method and gallic acid was used as comparative substance. Total phenolic content of ethanol extract was determinated by spectrophotometric method with Folin-Ciocalteu reagent quantitatively. Furthermore, the effective Scavenging 50 (ES50) value was calculated using linear regression between ethanol extract or galic acid concentration versus % of free radical scavenging. The ES50 values were statistically analysis with anova and LSDTest. Result.This study showed that ethanolextract of Ocimum sanctum seeds has antioxidant potency with ES50 value was 131.81 ìg/ml> ES50 of leaves (91.94 ìg/ml ) > ES 50of of gallic acid ( 3.54 ìg/ml)( p