Microscopic particle discrimination using spatially-resolved Fourier-holographic light scattering angular spectroscopy

We utilize Fourier-holographic light scattering angular spectroscopy to record the spatially resolved complex angular scattering spectra of samples over wide fields of view in a single or few image captures. Without resolving individual scatterers, we are able to generate spatially-resolved particle size maps for samples composed of spherical scatterers, by comparing generated spectra with Mie-theory predictions. We present a theoretical discussion of the fundamental principles of our technique and, in addition to the sphere samples, apply it experimentally to a biological sample which comprises red blood cells. Our method could possibly represent an efficient alternative to the time-consuming and laborious conventional procedure in light microscopy of image tiling and inspection, for the characterization of microscopic morphology over wide fields of view.SAA is supported by the Raine Medical Research Foundation. We gratefully acknowledge contributions to the work presented here by Guido Kudielka, Michael Same, and Abbey Trewenack.peer-reviewe