Simple characterization scheme for optical coherence tomography systems with application to a commercial and a near-isometric resolution fibre-based system

Optical Coherence Tomography (OCT) is a rapidly growing imaging modality in biomedical optics. OCT can perform high-resolution, cross-sectional imaging of the microstructure of biological tissues by measuring the coherent spectrum from the backscattered light. OCT systems with broad spectral bandwidths are often constructed using free-space optics to avoid dispersion by fibre optic components. This paper presents a fibre-based OCT system at a centrewavelength of 1300nmwith an axial resolution of 3.8 ¿m in air, surpassing any previously reported values to the best of our knowledge. Despite the challenges in transporting a broadband spectrum using fibre-optics, the system investigation was motivated by the ever-increasing demand for commercialization of high-resolution OCT systems and simplification of construction. We also evaluate and demonstrate the direct measurement method for axial resolution using an air wedge. Imaging of biomedical and other samples is demonstrated using a high numerical aperture sample lens and compared with images from a commercial OCT system. We discuss the effect of the improved structural visibility by achieving image voxels closer to an isometric shape with a high NA sample lens.European Union’s Horizon 2020 research and innovation programme (Grant Number: 761214 and 779960) IEEE Plagiarism Policypeer-reviewe