International audienceTo carry initiator Met-tRNA(i)(Met) to the small ribosomal subunit, eukaryal and archaeal cells use a heterotrimeric factor called e/aIF2. These cells also possess a homologue of bacterial IF2 called e/aIF5B. Several results indicate that the mode of action of e/aIF5B resembles some function of bacterial IF2. The e/aIF5B factor promotes the joining of ribosomal subunits. Moreover, there is genetic evidence that the factor participates in the binding of initiator tRNA to the small ribosomal subunit. However, up to now, an interaction between e/aIF5B and initiator tRNA was not evidenced. In this study, we use an assay based on protection of aminoacyl-tRNA against spontaneous deacylation to demonstrate that archaeal aIF5B...
International audienceThe heterotrimeric factor e/aIF2 plays a central role in eukaryotic/archaeal i...
The eukaryotic translation initiation factor 2 (eIF2) binds the methionyl-initiator tRNA in a GTP-de...
International audienceInitiation of translation in eukaryotes and in archaea involves eukaryotic/arc...
International audienceTo carry initiator Met-tRNA(i)(Met) to the small ribosomal subunit, eukaryal a...
International audienceA critical consequence of the initiation of translation is the setting of the ...
International audienceEukaryotic and archaeal initiation factors 2 (e/aIF2) are heterotrimeric prote...
In eukaryotes and in archaea late steps of translation initiation involve the two initiation factors...
The function of initiation factors in and the sequence of events during translation initiation have ...
The function of initiation factors in and the sequence of events during translation initiation have ...
Translation initiation universally occurs with accurate selection of the start codon that defines th...
International audienceThe heterotrimeric factor e/aIF2 plays a central role in eukaryotic/archaeal i...
The eukaryotic translation initiation factor 2 (eIF2) binds the methionyl-initiator tRNA in a GTP-de...
International audienceInitiation of translation in eukaryotes and in archaea involves eukaryotic/arc...
International audienceTo carry initiator Met-tRNA(i)(Met) to the small ribosomal subunit, eukaryal a...
International audienceA critical consequence of the initiation of translation is the setting of the ...
International audienceEukaryotic and archaeal initiation factors 2 (e/aIF2) are heterotrimeric prote...
In eukaryotes and in archaea late steps of translation initiation involve the two initiation factors...
The function of initiation factors in and the sequence of events during translation initiation have ...
The function of initiation factors in and the sequence of events during translation initiation have ...
Translation initiation universally occurs with accurate selection of the start codon that defines th...
International audienceThe heterotrimeric factor e/aIF2 plays a central role in eukaryotic/archaeal i...
The eukaryotic translation initiation factor 2 (eIF2) binds the methionyl-initiator tRNA in a GTP-de...
International audienceInitiation of translation in eukaryotes and in archaea involves eukaryotic/arc...