Draft Genome Sequence of Medusavirus Stheno, Isolated from the Tatakai River of Uji, Japan

“Medusaviridae” is a proposed family of large double-stranded DNA (dsDNA) viruses so far represented by a sole virus isolated from a hot spring. In the present study, we report the isolation and genome sequencing of a second member of this family, medusavirus stheno, discovered from a freshwater sample with an Acanthamoeba castellanii coculture