Monitoring of liberation tests for the release of clotrimazole from nanofibers

Charles University Faculty of Pharmacy in Hradec Kralove Department of Analytical Chemistry Candidate: Radovan Ernest Supervisor: Doc. PharmDr. Hana Sklenarova, Ph.D. Consultant: PhamDr. Martina Hakova, Ph.D. Title of the diploma thesis: Monitoring of liberation tests for the release of clotrimazole from nanofibers The diploma thesis was focused on the analysis of release profiles of clotrimazole from different types of nanofibres using non-separation flow technique, sequential injection analysis (SIA). Simultaneously the diploma thesis dealt with finding ideal conditions for saturation of the fibres with a solution of clotrimazole. Nanofibers were produced at the Technical university of Liberec. The diploma thesis dealt with two types of nanofiber carriers - polymeric (polydioxanone and polycaprolactone) and ceramic (silica). Part of the fibre of polydioxanone and polycaprolactone was saturated directly during production in different ratios of polymer and clotrimazole. The saturated fibres were then analysed in the laboratory. The second part of polydioxanone and polycaprolactone together with ceramic nanofibers was produced in a pure state, i.e., without an active substance. These fibres were tested in the laboratory for saturation with an ethanolic solution of clotrimazole at a certain..