The effect of flubendazole on temozolomide treatment in glioblastoma cells

Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Kateřina Dvořáková Supervisor: prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The effect of flubendazole on temozolomide treatment in glioblastoma cells Glioblastoma multiforme (GBM) is one of the most common and aggressive brain tumors in adults. Despite significant advances in treatment, GBM remains to have very poor prognosis and median survival of only 12 to 15 months after diagnosis. Standard treatment consists of maximal possible surgical resection followed by chemo-radiotherapy with antitumor drug temozolomide (TMZ), which use is problematic due to fast developing chemoresistance. The aim of this study was to examine the effect of potential anticancer drug, anthelmintic flubendazole (FLU) and effect of FLU in combination with TMZ on GBM cells. For this purpose, two GBM cell lines were used - A172 and T98G. In general FLU reduced cell proliferation more, especially in T98G cells. Moreover, the use of different combinations of TMZ + FLU showed even higher inhibitory effect on the viability of GBM cell lines. The combination of TMZ + FLU reduced the protein level of -tubulin and III-tubulin, simoultaneously interesting changes in STAT3 and EGFR expression, as well as lower..