Biological activity of macromycetes - A

Zvonková, L. Biological activity of macromycetes - A. Charles University in Pragu, Faculty of Pharmacy in Hradec Králové, 96 pp. Key words: mushrooms, compounds, free radicals, thin-layer chromatography (TLC), antioxidant activity, sequential injection analysis (SIA) Fourteen different mushrooms taxons (orders − Boletales, Pezizales, Polyporales and Russulales) were examined in this diploma thesis. Lyophiziled extracts were prepared under the precise set of conditions from the fruiting bodies and consequently used for recognition of compounds groups and for antioxidant activity detection. Thin-layer chromatography (TLC) was used for the determination of compounds groups. This TLC process proceeded in ascending development in pre-saturated chambers. From TLC results it was detected that tested mushrooms extracts contained especially amino-acids, saccharides, carboxylic acids, phenolic substances, sterols, which corresponds with the available literature. Alkaloids were also detected, especially in Leccinum griseum (Quél.) Singer extract. Antioxidant activity was set using sequential injection analysis method (SIA), which is based on DPPH radical bleaching. This method is very suitable, due to its rapidness and sensitivity, for evaluation of multiplicity routine antioxidant activity screenings. The..