The Effect of Elicitation on Production of Secondary Substances in Cultures in vitro

The effect of elicitation on production of secondary substances in cultures in vitro Plant secondary metabolites are important sources for pharmaceutical, chemical and food industry. Elicitation is one of the possibilities how to increase the production of secondary subtances in cultures in vitro. The effect of abiotic elicitor 2- (3-brombenzylsulfanyl)pyridine-4-karbothioamide with antituberculotic activity on the isoflavonoids production Genista tinctoria callus and suspension culture was tested in this study. The elicitor was applied in three concentrations. Elicitation was run in this time intervals: 6, 12, 24, 48, 72 and 168 hours. Cultures were cultivated on nutrient medium, that described Shenk and Hildebrant with the addition of 0,5 mg/l of 2,4- dichlorfenoxyacetic acid and 0,1 mg/l of kinetin. The content of isoflavonoids was determined by HPLC. The highest content of isoflavonoids (0,03 %) in callus culture was found after the application of elicitor's solution in concentration of c3 = 2,96 x 10-5 mol/l after 24 hours of treatment. The maximum production of daidzein (0,02 %) in callus culture was detected after 48 hours of elicitor treatment in concentartion of c1 = 2,96 x 10-3 mol/l and after 24 hours of elicitor treatment in concentration of c3 = 2,96 x 10-5 mol/l. Suspension culture..