Determination of radiochemical purity and stability of new radiopharmaceuticals

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Nikola Vorlová Supervisor: Prof. PharmDr. Ing. Milan Lázniček, CSc. Title of diploma thesis: The determination of radiochemical purity and stability of newly developed radiopharmaceuticals. The theme of this thesis is determination of radiochemical purity and stability of newly developed radiopharmaceuticals from the group of receptor specific peptides and antibodies by using liquid chromatography - HPLC and thin layer chromatography ITLC -SG method. The antibody sample 177Lu-hR3-I has been studied in the theses( 177 Lu enters in to relation with modified antibody Nimotuzumab), which blocs EGF receptors and due to this fact the growth of tumor cells too. It has been detected, that it is necessary for reaching sufficient radiochemical purity to clean this radio-labeled antibody by the gel chromatography after labeling. Radio-chemical purity of this sample circulate from 99,46 % to 88,77 % during period of 6 days. Another samples have been studied, 111In-minigastrin - radioisotope 111 In linked up to three different minigastrins I, II, and III. Minigasrins are gastrin derivates , which have a very good absorptive capacity to receptors for cholecystokinin (CCK-B receptors) occurring in..