Evaluation of biologically active substances using HPLC IV.

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department Pharmaceutical Chemistry and Drug Kontrol Name of Degrese Paper: Evaluating of biologically active substances by HPLC IV. Candidate: Martina Seidlová Tutor: PharmDr. Pavla Pilařová, Ph.D. In this diploma work the study of dilation behaviour of medications was provided under extreme conditions for a column being able to measure pH ranged 1-12 on column Kinetex EVO C18 (150 x 3 mm). For higher pH the following medications were studied: quetiapine, and its two metabolites - norquetiapine and 7-hydroxyquetiapine, bromhexine, codeine, zolpidem and its admixture, fluoxetine and aciclovir. The best results were reached with quetiapine with its metabolites, therefore they were chosen for the following tests and possibility of separation from plasma. As a mobile phase an acidic buffer was used at pH10 using ratio 70:30 to organic phase which was made of acetonitrile and methanol (55:5). For lower pH the following medications were studied: salicylic acid, acetylsalicylic acid, aciclovir, bromhexine and fluoxetine. The best results were reached with salicylic acid and acetylsalicylic acid, therefore they were chosen for the following testing and the possibility of separation in the medicament. As a mobile phase a phosphate buffer..